Dualex植物多酚-叶绿素仪：近端植物传感器在菊花施肥中的应用

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Dualex植物多酚-叶绿素仪：近端植物传感器在菊花施肥中的应用

发表时间：2022-04-27 10:39:53点击：816

来源：北京博普特科技有限公司

Dualex是一款源自于法国国家科学院 (CNRS)及巴黎第十一大学技术，由奥地利PESSL公司生产（原法国Force-A公司）开发的新型多功能叶片测量仪。它可同时准确测量叶片的叶绿素含量、叶片表层的类黄酮和花青素含量，适用于植物生理学和农学（如水稻叶绿素浓度，玉米氮素状况，葡萄藤等）相关研究。

其测量对象可以是单子叶植物，双子叶植物或多年生植物。这款设备简单易用，可进行实时和非破坏性测量。由于不需要校准标定和事先的样品制备，测量工作可在实验室或现场完成。此外，该设备在各种温度的和环境光照条件下均可正常使用。系统采用专利设计，光学传感器可进行简单、快速、无损测量叶片中的叶绿素、多酚以及花青素。

Dualex便携式智能气象-叶绿素-花青素-黄酮醇测量仪

热点

植物传感器可以帮助种植者和顾问减少菊花中的氮损失。

对于叶片水平的传感器，包括LMA将改善与N的相关性。

用Dualex测定的表皮多酚是叶面N的最佳替代物。

NDVI与生物量相关。

摘要

菊花是全世界普遍种植的观赏植物。适当的氮肥供应时机对于优质作物和种植者的投资回报至关重要。次优氮营养对商品植物的品质有负面影响，而超优氮营养由于硝酸盐淋失而对环境有影响。因此，需要（a）工厂氮状态的可靠指示器。连续三年（2016年至2018年）对两个大田盆栽菊花品种“玛雅”和“奥兰多”进行了研究。为了获得氮含量的变化，采用了三种不同的氮处理。植物质量测量包括株高、直径、单位面积叶质量（仅2017年和2018年）、生物量、叶片和植株氮含量分析。使用SPAD传感器（2016年和2017年）和Dualex Scientific传感器（2017年和2018年）在叶片水平上进行光学测量，使用GreenSeeker NDVI仪在冠层水平上进行光学测量。最小施肥处理的生物量、高度和直径均较小。单位面积叶质量对SPAD和Dualex测定的氮和叶绿素之间的关系有影响。与非破坏性叶绿素测量和氮平衡指数相比，Dualex测量的表皮多酚与叶片氮的相关性更好。由于远轴表皮多酚与叶片氮含量高度相关，且便于田间测定，因此我们提出该测定方法为菊花施肥提供决策支持。由于品种的多样性，有时是逐年变化的，参考地块可以为种植者和顾问提供帮助。研究发现，NDVI更容易受到年度变化的影响，但与几个质量参数的高度相关性和使用的便利性使该植被指数有助于检测空间质量变化的程度，从而支持在生长季结束时达到所需植株直径的地点依赖性N需求。

Utility of proximal plant sensors to support nitrogen fertilization in Chrysanthemum

Highlights

Plant sensors can help growers and advisors to reduce N losses in Chrysanthemum.

For sensors at leaf level, including LMA will improve correlations with N.

Epidermal polyphenol measured with Dualex were the best proxy for foliar N.

NDVIis correlated with biomass.

Abstract

Chrysanthemum morifolium Ramat. is a commonly grown ornamental worldwide. A proper timing of nitrogen (N) supply is essential for a qualitative crop and the return on investment for growers. Sub-optimal nitrogen nutrition negatively influences the commercial plant quality, while supra-optimal N has an environmental impact due to nitrate leaching. Therefore, (a) reliable indicator(s) of plant nitrogen status is/are needed. Two field-grown potted Chrysanthemum cultivars, ‘Maya’ and ‘Orlando’ were studied for three consecutive years (2016–2018). Three different N treatments were applied in order to obtain a variation in N content. Plant quality measurements consisted of plant height, diameter, leaf mass per area (2017 and 2018 only), biomass and foliar and plant N content analysis. Optical measurements were performed with a SPAD sensor (2016 and 2017) and a Dualex Scientific sensor (2017 and 2018) on leaf level and with a GreenSeeker NDVI meter on canopy level. Biomass, height and diameter tended to be smaller in the minimal fertilizer treatments. Leaf mass per area did influence the relation between N and chlorophyll measured with SPAD and Dualex. Epidermal polyphenolics measured with Dualex correlated better with foliar nitrogen than non-destructive chlorophyll measurements and the nitrogen balance index. Since abaxial epidermal polyphenolics were highly correlated with foliar nitrogen and convenient to measure in-field, we propose this measurement for decision support in Chrysanthemum fertilization. Because of cultivar and sometimes year-to-year variability, reference plots can be of help for growers and advisors. NDVI was found to be more susceptible for yearly variation, but very high correlation with several quality parameters and convenience in use make this vegetation index useful for detecting the extent of spatial quality variability and thus support site dependent N requirements to reach the desired plant diameter at the end of the growing season.