摘要

   在玉米育种项目中使用玉米的双倍体（DHs）越来越普遍，在短短的2年时间里，育种人员就可从杂交实验阶段进入到评估阶段。用于玉米的体内DH系统有两个重要特点：(i) 鉴别单倍体后代(ii)将单倍基因组翻倍形成自交系。研究主要聚焦于先进步。

   目前鉴别玉米后代主要是人工通过R1-nj 种子颜色标记。该过程耗时耗力；自动筛选单倍体的方法将较大提升DH品系研发效率。

   此研究中，6个带有母系单倍体诱导物‘RWS/RWK-76’的自交系玉米进行杂交，每个品系的种子样品手工筛选。使用VideometerLab 3 多光谱成像系统来区分单倍体和杂交体。使用DNA标记来确定检测种子的单倍体/双倍体状态，大多数基因型的单倍体鉴别成为可能，精度较过50%。详细产品介绍请参见VideometerLab 产品链接。

Discrimination of haploid and diploid maize kernels via multispectral imaging

Abstract

The use of doubled haploids (DHs) in maize has become ubiquitous in maize breeding programmes as it allows breeders to go from cross to evaluation in as little as 2 years. Two important aspects of the in vivo DH system used in maize are as follows: (i) the identification of haploid progeny and (ii) doubling of the haploid genome to produce fertile inbred lines. This study is focused on the first step.

 Currently, identification of maize haploid progeny is performed manually using theR1-nj seed colour marker. This is a labour-intensive and time-consuming process; a method for automated sorting of haploids would increase the efficiency of DH line development.

In this study, six inbred lines were crossed with the maternal haploid inducer ‘RWS/RWK-76’ and a sample of seed was sorted manually for each line. Using the VideometerLab 3 system, spectral imaging techniques were applied to discriminate between haploids and hybrids. Using DNA markers to confirm the haploid/diploid state of the tested seed, for the majority of genotypes haploid identification was possible with over 50% accuracy.