Dualex植物多酚-叶绿素仪：硅在渗透胁迫下介导两种不同番茄基因型差异耐逆反应中的调节作用

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Dualex植物多酚-叶绿素仪：硅在渗透胁迫下介导两种不同番茄基因型差异耐逆反应中的调节作用

发表时间：2022-05-05 13:38:52点击：1183

来源：北京博普特科技有限公司

Dualex是一款源自于法国国家科学院 (CNRS)及巴黎第十一大学技术，由奥地利PESSL公司生产（原法国Force-A公司）开发的新型多功能叶片测量仪。它可同时准确测量叶片的叶绿素含量、叶片表层的类黄酮和花青素含量，适用于植物生理学和农学（如水稻叶绿素浓度，玉米氮素状况，葡萄藤等）相关研究。

其测量对象可以是单子叶植物，双子叶植物或多年生植物。这款设备简单易用，可进a行实时和非破坏性测量。由于不需要校准标定和事先的样品制备，测量工作可在实验室或现场完成。此外，该设备在各种温度的和环境光照条件下均可进行简单、快速、无损测量叶片中的叶绿素、多酚以及花青素。

Dualex便携式智能气象-叶绿素-花青素-黄酮醇测量仪

摘要

此前的研究表明，硅（Si）在减轻干旱胁迫对不同作物物种的不利影响方面发挥了作用。然而，缺乏关于耐旱和抗旱作物品种对硅营养反应的比较数据。因此，本研究旨在阐明渗透胁迫条件下两种不同番茄基因型对硅营养的反应机制。在聚乙二醇（PEG，6000）和两种硅（0和1.5mm）条件下水培生长了两个对干旱胁迫反应不同的番茄品系。代谢物分析分两行进行。还测量了生长和相关生理参数，以及所选基因的表达水平。施硅提高了耐旱系LA0147和干旱敏感系FERUM的渗透胁迫耐受性。在耐旱系中，硅提高了硫（S）和铵（NH+4NH4+）的吸收，从而显著提高了氨基酸精氨酸、蛋氨酸、丝氨酸和甘氨酸的产量。而在干旱敏感品系中，Si显著增加了氨基酸脯氨酸和GABA的产生，从而进一步降低了GSSG与GSH的比例，从而平衡了渗透胁迫下的氧化还原稳态。氨基酸精氨酸、蛋氨酸、GABA和脯氨酸的大量产生促进了游离多胺腐胺和亚精胺的产生，并提高了渗透胁迫耐受性。因此，我们得出结论，Si通过相关氨基酸的差异积累，在两个不同的番茄基因型中显著调节渗透胁迫耐受性，最终导致多胺代谢增强。

关键词：氨基酸；代谢物；矿物质营养和氧化还原状态；多胺；番茄

Regulatory Role of Silicon in Mediating Differential Stress Tolerance Responses in Two Contrasting Tomato Genotypes Under Osmotic Stress

Abstract

Previous studies have shown the role of silicon (Si) in mitigating the adverse effect of drought stress in different crop species. However, data are lacking on a comparison of drought tolerant and drought sensitive crop cultivars in response to Si nutrition. Therefore, the aim of this study was to elucidate the mechanism (s) by which two contrasting tomato genotypes respond to Si nutrition under osmotic stress condition. Two tomato lines contrasting in their response to drought stress were hydroponically grown under polyethylene glycol (PEG, 6000) and two regimes of Si (0 and 1.5 mM). Metabolite profiling was performed in two lines. Growth and relevant physiological parameters, and expression levels of selected genes were also measured. Si application resulted in improved osmotic stress tolerance in both drought tolerant line LA0147 and drought sensitive line FERUM. In the drought tolerant line, Si enhanced uptake of sulfur (S) and ammonium ( NH+4NH4+ ) which led to a significantly higher production of amino acids arginine, methionine, serine, and glycine. While in the drought sensitive line, Si significantly increased production of amino acids proline and GABA which further lowered the level of GSSG to GSH ratio and thus balanced the redox homeostasis under osmotic stress. The higher significant production of amino acids arginine, methionine, GABA, and proline enhanced production of free polyamines putrescine and spermidine and improved osmotic stress tolerance. Therefore, we conclude that Si distinctively regulated osmotic stress tolerance in two contrasting tomato genotypes by differential accumulation of relevant amino acids which eventually led to enhanced polyamine metabolism.

Keywords: amino acids; metabolites; mineral nutrition and redox state; polyamines; tomato.