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利用Shutter水下自开合叶绿素荧光测量系统发表海藻研究文章
发表时间:2019-02-14 13:17:04点击:1690
来源:北京博普特科技有限公司
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Shutter水下自开合叶绿素荧光测量系统是Aquation主打叶绿素荧光测量系统,水陆两用,长期值守,全自动开合叶室,程序控制叶室闭合进行暗适应测量,测量ΦII, FV/FM, PAR和温度,快门实现叶绿素荧光诱导曲线、NPQ弛豫和RLC(快速光曲线),无人值守自动监测,自动增益和自动归零功能:自动在野外进行正确设置,数据采集器可同时操作多个传感器,简单开关启动水下或陆地测量程序,全防水可达50m,潜水坚固不锈钢或工程塑料设计,扩展大型外壳与电池包,利用易用软件选择所供程序或设定程序,根据程序,可自动运行达72h,开合型传感器可通过电脑控制,用于预田间实验,增加数采可以扩展到多个传感器(同时测量可达15个)。相关科研人员发表了Distinguishing downregulation from other non-photochemical quenching of an Antarctic benthic macroalga using in situ fluorometry。通过该技术,科研人员打开了精细研究调控和原位光合动力学以及相关非光化学过程的大门。研究中使用了Shutter水下自开合叶绿素荧光测量系统。
图1.定制荧光传感器
(a) Sidefire 传感器,Iridaea sp. thallus安装在传感器左侧和下方
(b) Shutter传感器关闭
(c)Shutter传感器打开,PAR传感器盘,箭头指示测量样品位置。
图2.PSII和 Fv/Fm( Iridaea sp.),几天内在 Molholme 岛进行原位测量。原位辐照度见下方图像。数据来自单个代表性 Iridaea sp.巨藻样本。
北京博普特科技有限公司是澳大利亚Aquation公司中国区代理,全面负责其系列产品在中国市场的推广、销售和售后服务。论文摘要参见下文。
The quantum yield of chlorophyll a fluorescence of an Antarctic macroalga, Iridaea cordata, was measured in situ using logging fluorometers with an automated dark-acclimation capability throughout several diel periods. Application of far-red light and dark acclimation enabled the automated determination of photochemical quenching and non-photochemical quenching parameters that have until recently been restricted to manual measurement in terrestrial and shallow water envi- ronments. Our results show that non-photochemical quenching processes in Iridaea thalli that have been exposed to mod- erately high irradiances during the day, take all night to relax. We also show that, for algae exposed to relatively high light during the day, around 30–40% of total excitation flux is allocated to basal intrinsic non-radiative decay processes, with the remainder allocated to downregulation or photochemistry. The ability to evaluate the allocation of energy to these funda- mentally different non-photochemical quenching processes has direct applications in assessing algal responses to both natural and anthropogenic stress. With this technology we open the door to a far more detailed examination of the regulation and kinetics of in situ photosynthesis and associated non-photochemical processes.
Key words: Antarctica, chlorophyll fluorescence, dark acclimation, in situ, Iridaea sp., irradiance, macroalgae, non-photochemical quenching