Dualex植物多酚-叶绿素仪：用非破坏性荧光传感器监测两个苹果品种对供水条件的生理生化反应

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Dualex植物多酚-叶绿素仪：用非破坏性荧光传感器监测两个苹果品种对供水条件的生理生化反应

发表时间：2022-05-05 13:41:41点击：1328

来源：北京博普特科技有限公司

Dualex是一款源自于法国国家科学院 (CNRS)及巴黎第十一大学技术，由奥地利PESSL公司生产（原法国Force-A公司）开发的新型多功能叶片测量仪。它可同时准确测量叶片的叶绿素含量、叶片表层的类黄酮和花青素含量，适用于植物生理学和农学（如水稻叶绿素浓度，玉米氮素状况，葡萄藤等）相关研究。

其测量对象可以是单子叶植物，双子叶植物或多年生植物。这款设备简单易用，可进a行实时和非破坏性测量。由于不需要校准标定和事先的样品制备，测量工作可在实验室或现场完成。此外，该设备在各种温度的和环境光照条件下均可进行简单、快速、无损测量叶片中的叶绿素、多酚以及花青素。

Dualex便携式智能气象-叶绿素-花青素-黄酮醇测量仪

热点

Chl荧光技术评估植物生理反应的适用性。

叶绿素浓度不是一个合适的水分胁迫指标。

品种‘Pinova 10’对叶绿素荧光表现出较高的水分亏缺敏感性。

叶片水势特别受提前浇水制度的影响。

摘要

在世界范围内，水资源短缺导致的干旱胁迫加剧了保持农业和园艺作物的高产的危险性。众所周知，不同的作物不仅需要不同的水分，而且不同的品种也需要不同的水分。最常见的是，植物对干旱胁迫的反应是在生理水平上监测的，例如通过植物水分状况（叶水势、相对含水量）或气孔行为、光合作用和渗透调节。然而，对这些参数的分析既耗时又具有破坏性。相比之下，荧光传感器等新方法似乎很有前途，因为它们易于在现场进行，无破坏性且分析迅速。因此，本研究的目的是调查两个苹果品种“Pinova 10”和“Gala Galaxy”对水分胁迫是否表现出不同的生理反应，例如叶片水势、相对含水量以及叶绿素和脯氨酸浓度。此外，叶绿素荧光参数被认为是一种很有前途的应激症状检测技术。“Gala Galaxy”比“Pinova 10”表现出更高的耐水性，尤其是在整个实验过程中的某些测量日，叶绿素荧光指数增加。与叶绿素含量（Chl_指数和SFR_R）和氮平衡（NBI和NBI_R）相关的荧光指数显示出相似的曲线。然而，用湿化学方法进行的叶片叶绿素分析并不是苹果树水分胁迫的可靠指标。在完全停水期间，DAT 38的叶水势受到影响，品种间没有显著差异。综上所述，与叶绿素含量和氮平衡相关的荧光指数有望成为一种有用的非破坏性工具，用于评估受水分限制的幼树的生理状态。

Monitoring physiological and biochemical responses of two apple cultivars to water supply regimes with non-destructive fluorescence sensors

Highlights

Suitability of Chl-fluorescence technique to assess plant physiological responses.

Chlorophyll concentration was not an appropriate water stress indicator.

Cultivar ‘Pinova 10’ showed higher sensibility to water deficit on Chl-fluorescence.

Leaf water potential is specially affected advanced watering withholding regime.

Abstract

Worldwide, increased drought stress induced by water shortages will be especially hazardous for maintaining high yields in agricultural and horticultural crops. It is known that not only do different crops have different water requirements, but that these can also vary between cultivars. Most commonly, plant responses to drought stress are monitored on the physiological level, for example via plant water status (leaf water potential, relative water content) or stomatal behavior, photosynthesis and osmotic adjustment. However, analyses of these parameters are time-consuming and destructive. In contrast, novel approaches such as fluorescence sensors seem promising as they are easy to embark in the field, non-destructive and quick to analyze. Therefore, the aim of this study was to investigate whether two apple (Malus domestica) cultivars, ‘Pinova 10’ and ‘Gala Galaxy’, show different physiological reactions, for example in leaf water potential, relative water content as well as chlorophyll and proline concentration, in response to water stress. In addition, chlorophyll fluorescence parameters were analyzed as a promising stress symptom detection technique. ‘Gala Galaxy’ showed higher tolerance to water deprivation conditions than ‘Pinova 10’, indicated especially by increased chlorophyll fluorescence indices on certain measurement days throughout the experimental course. Fluorescence indices related to chlorophyll content (Chl_Index and SFR_R) and nitrogen balance (NBI and NBI_R) showed similar curves. However, leaf chlorophyll analysis performed wet-chemically was not a reliable indicator of water stress in apple trees. Leaf water potential was affected on DAT 38, during complete watering withholding, without significant differences between the cultivars. In summary, fluorescence-based indices, related to chlorophyll content and nitrogen balance, promise to be a useful non-destructive tool to estimate physiological status of young apple trees submitted to water restriction regimes.