摘要

萌发期的低温影响了萌发速度以及早期苗发育。Zn种子引发是有用、性价比高的工具，用来提升萌发和早期苗发育时期的低温发芽速率和抗性。我们对菠菜种子进行了检测，在低温胁迫条件下，利用Zn引发提升萌发和苗发育。Zn引发提升种子Zn浓度达48倍。VideometerLab多光谱成像设备作为无损方法成功区分了未引发、水和Zn引发种子。Zn在种子中的定位研究使用了1,5-diphenyl thiocarbazone (DTZ)染色技术。最后利用激光共聚焦显微镜检测幼苗根内引发Zn的活跃1转运。Zn 引发（6 mM）菠菜种子表明在低温8℃条件下，其萌发速率和总萌发显著增加。.

Zinc Seed Priming Improves Spinach Germination at Low Temperature

Muhammad Imran，, Asim Mahmood, Günter Neumann  and Birte Boelt

Abstract: Low  temperature during  germination  hinders germination  speed and early seedling development. Zn seed priming is a useful and cost-effective tool to improve germination rate and resistance to low temperature stress during germination and early seedling development. Spinach was tested to improve  germination  and seedling development with  Zn seed priming under low temperature stress conditions.  Zn priming increased seed Zn concentration up to 48 times. The multispectral imaging technique with  VideometerLab was used as a non-destructive method to differentiate unprimed, water- and Zn-primed spinach seeds successfully. Localization of Zn in the seeds was studied using the 1,5-diphenyl thiocarbazone (DTZ) dying technique. Active translocation of primed  Zn in the roots of young seedlings was detected with  laser confocal microscopy.  Zn priming of spinach seeds at 6 mM Zn showed a significant increase in germination rate and total germination under low temperature at 8 ◦ C.

Keywords: spinach; Zn priming; multispectral imaging; Zn localization; abiotic stress